FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573876
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10700.030.7670732
TACTCGG10800.029.1544443
CGAGTAG11500.028.6057873
CCGAGTA11950.028.3358692
CCCGAGT15700.027.342991
GCTGGGA99500.027.2070589
GCTACTC28600.026.7210331
GGAGGCT68500.026.003449
CCCAAAG74450.025.9790571
GAGTAGC30100.025.9176314
AGTAGCT46600.024.0018525
TCGGGAG16250.023.7145126
CTCGGGA14700.023.018075
CCAAAGT73350.023.017932
TAGCTGG53800.022.8002957
GTAGCTG50850.022.3654756
GGGAGGC78550.022.2576318
TTGGGAG64800.021.829576
AGTGCTG70050.021.669466
GTGCTGG70050.021.6037887
Produced by FastQC (version 0.11.5)