FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573876
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG12200.033.9246032
CGAGTAG10950.032.64313
TACTCGG12400.031.8602753
CCGAGTA11450.031.6283952
GCTACTC30700.031.2582421
TCGCGTT1252.3518078E-730.1003765
CCCGAGT14700.029.1203611
CGGAGGT17500.028.740887
GCTGGGA97550.027.1264369
GGAGGCT79550.026.8833129
CCCAAAG76250.026.2193411
CTCGGGA15850.026.1123125
GAGTAGC31050.026.053064
AGTAGCT47600.025.7884655
TCGGGAG17100.025.5683636
GTAGCTG49050.025.0159846
CTCGCGT1901.3254976E-824.7535974
GCGGAGG22650.024.4923216
TTGGGAG72850.024.4582716
TAGCTGG50550.024.45627
Produced by FastQC (version 0.11.5)