FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407388
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15950.048.0266273
CCGAGTA16900.044.522722
CCCGAGT19200.042.233891
CTACTCG13100.041.283352
AGTAGCT59200.041.1209535
TACTCGG12600.041.027713
AAAGTGC58500.040.7293244
CCCAAAG72700.040.400571
GAGTAGC37600.040.246044
AAGTGCT61300.040.095575
GCTGGGA124200.040.0721139
GTAGCTG60900.039.818746
CCAAAGT67650.038.581012
CAAAGTG65350.038.0421643
TAGCTGG66350.037.8948977
AGTGCTG69050.035.5272836
GCTACTC27650.035.124161
GTGCTGG74100.033.2338757
GGAGGCT89800.032.9186639
TTGGGAG74750.032.6924366
Produced by FastQC (version 0.11.5)