FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998871
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9100.043.9247932
CGAGTAG12400.040.1736343
TACTCGG9650.039.9340633
CCCAAAG61150.039.394681
AGTAGCT51650.039.125075
CCGAGTA12650.039.0329172
AAAGTGC52850.038.1477784
GCTGGGA106850.037.958389
AAGTGCT54250.037.769715
GAGTAGC31500.037.4472664
CCAAAGT60650.036.5192872
GTAGCTG55350.036.424756
TAGCTGG58300.036.033837
CAAAGTG60350.035.5873683
GCTACTC23500.035.3067671
CCCGAGT15650.033.737811
AGTGCTG59950.033.708256
TTGGGAG61950.032.9993136
GTGCTGG61400.032.5305987
GGAGGCT73950.032.4118239
Produced by FastQC (version 0.11.5)