FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998871
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11150.046.400763
CCGAGTA11700.045.4255182
GAGTAGC28400.044.3829844
AGTAGCT48700.043.7491155
GCTGGGA105900.041.9860849
GTAGCTG49300.041.8642856
CTACTCG11350.041.4392052
CCCAAAG69100.040.9831661
AAAGTGC56350.040.9814764
TACTCGG11950.040.145753
CCAAAGT65500.039.278362
TAGCTGG54100.039.272417
TTGGGAG68150.039.046016
AAGTGCT60200.038.9855885
AGTGCTG60900.038.290456
CAAAGTG63350.038.23563
CCCGAGT14950.037.4449121
GTGCTGG64650.036.5717627
GGAGGCT79850.034.198849
TTTGGGA63900.033.4160425
Produced by FastQC (version 0.11.5)