FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702593
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11450.042.713453
CGAGTAG11550.040.715043
CTACTCG12650.039.033332
CCGAGTA12300.037.850092
GCTACTC29450.037.3748051
GCTGGGA106150.036.7945149
CCCAAAG74050.035.1911161
AAAGTGC61950.034.5383034
GGAGGCT82650.034.347589
CCAAAGT69050.034.1882482
GAGTAGC29600.034.156844
TTGGGAG75550.033.7901886
AGTAGCT47550.033.5258755
AAGTGCT67050.032.26195
CCCGAGT15650.032.1601261
CTCGGGA16550.031.539585
CAAAGTG72700.031.5015093
GTGCTGG67600.031.5001227
GTAGCTG51100.031.281196
AGTGCTG67700.031.1110466
Produced by FastQC (version 0.11.5)