FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354076
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12950.042.4597633
TACTCGG10350.041.7742733
CCGAGTA13500.040.4089052
CTACTCG10750.039.3719522
GCTGGGA104750.038.4044159
AGTAGCT50200.036.7917335
GAGTAGC29850.036.6836934
CCCAAAG70100.035.5917361
AAAGTGC58250.035.3378074
CCCGAGT16750.035.1970861
CCAAAGT63300.034.8435172
GCTACTC25600.034.636131
GTAGCTG53150.034.5728266
AAGTGCT60350.034.1860285
TAGCTGG56550.034.1562847
TTGGGAG66250.032.6312266
CAAAGTG66400.032.4867323
AGTGCTG62850.032.4523286
GTGCTGG65600.031.1635447
TCGGGAG15200.030.6092746