FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354076
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12700.045.9258772
CGAGTAG12400.045.1403353
TACTCGG12850.042.095363
CTACTCG13200.041.6918752
GAGTAGC32100.040.4424174
GCTACTC26900.040.40081
GCTGGGA107150.038.4717869
AGTAGCT51450.037.8484425
AAAGTGC58400.037.6934624
CCCAAAG73250.037.4447751
CCAAAGT67000.037.138162
AAGTGCT60550.036.898825
GTAGCTG53200.036.4140976
TTGGGAG71150.035.752496
AGTGCTG63000.035.7502376
CCCGAGT16950.035.250421
TCGGGAG16500.035.051386
TAGCTGG56250.034.851887
CAAAGTG67200.034.7877733
GGAGGCT81950.033.6111689
Produced by FastQC (version 0.11.5)