FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3915465
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11100.033.4693872
CGAGTAG11050.033.1952483
TACTCGG12150.032.5122223
CTACTCG12750.030.6133982
GGAGGCT75450.029.5299649
GCTGGGA96050.028.5798079
GAGTAGC27350.028.5411974
CCCGAGT15200.028.1605661
AGTAGCT46350.028.0011065
GCTACTC29600.027.809291
TTGGGAG64900.027.7451256
GTAGCTG45800.027.5107176
TAGCTGG50900.026.8748977
CCCAAAG66600.026.7675461
GGGAGGC90250.026.1977868
CGGAGGT15150.026.063837
AAAGTGC55250.025.7037264
CCAAAGT64900.024.6364352
AAGTGCT59150.024.5651595
AGTGCTG61200.024.505976
Produced by FastQC (version 0.11.5)