FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484420
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA14800.045.435542
CGAGTAG14200.044.6791573
CCCGAGT17800.042.110571
CTACTCG10950.041.2266922
TACTCGG11150.040.8842853
AGTAGCT50150.038.046495
GTAGCTG51550.037.1955536
GCTGGGA99600.037.0409669
GAGTAGC33150.037.001264
TAGCTGG53150.036.695847
GCTACTC26550.033.5591741
CCCAAAG69800.032.5540921
AAGTGCT56150.032.390735
CCAAAGT63450.032.1646962
AAAGTGC55900.032.0311624
TCGGGAG15700.032.029026
GGAGGCT72850.031.9335829
GTGCTGG60050.031.6966977
TTGGGAG64800.031.6206656
AGTGCTG59650.031.4356166
Produced by FastQC (version 0.11.5)