FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511959
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9700.036.848272
TCGCGTT906.1993524E-736.5523535
TACTCGG9550.036.415663
GCTGGGA88500.032.5524189
CCGAGTA11350.032.32022
AGTAGCT44750.032.0307125
CGAGTAG10650.031.7718643
GTAGCTG45200.031.0879886
GCTACTC23200.030.4785651
GAGTAGC28100.030.1041284
CCCAAAG63950.029.1170771
TAGCTGG50100.028.6106787
AAGTGCT54950.027.1113645
CCAAAGT61650.027.0813662
AAAGTGC54400.026.9535224
GGAGGCT64750.026.9277579
CTCGGGA13200.026.7022085
TCGGGAG14450.026.6689476
CCCGAGT14400.026.5163521
CAAAGTG62100.025.579083
Produced by FastQC (version 0.11.5)