FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511959
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11850.036.1176832
TACTCGG11750.036.024793
CCGAGTA11650.035.5265922
GAGTAGC27200.034.235824
CGAGTAG11050.034.0505833
TTGGGAG68000.032.4946986
GCTACTC27950.032.481361
AGTAGCT42300.031.9099375
CCCAAAG66500.031.9016861
GCTGGGA91000.031.7629769
GGAGGCT73900.031.3537839
GTAGCTG43500.030.8020176
CCCGAGT14350.030.485251
AAAGTGC55650.029.6637734
GGGAGGC92050.029.6140528
CGGAGGT15350.029.3976217
AACGCGC804.7831322E-429.3747168
TAGCTGG48150.029.0917117
CTCGGGA15550.029.0352295
CAAAGTG63350.028.5089633
Produced by FastQC (version 0.11.5)