FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203028
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11100.049.5358923
CTACTCG11350.048.4765782
CCGAGTA11550.048.0443152
CGAGTAG11400.047.8200723
GAGTAGC30250.042.7232674
AGTAGCT49850.041.951935
GCTACTC27150.041.318131
GCTGGGA107900.040.7678959
CCCGAGT14600.040.3543781
GTAGCTG52450.040.1411326
CCCAAAG70300.040.0269551
AAAGTGC59400.039.7168124
AAGTGCT60200.039.1109475
TCGGGAG16000.038.7713626
AGTGCTG62300.038.1697736
CCAAAGT67250.038.0407032
CAAAGTG64800.037.204843
TAGCTGG56350.037.1133357
GGAGGCT76750.036.311179
ACTCGGG16000.035.5404174
Produced by FastQC (version 0.11.5)