FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203028
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13200.051.2979972
TACTCGG13400.051.234193
CCGAGTA14550.048.8006672
CGAGTAG14750.046.863763
GCTACTC31100.046.7258721
AGTAGCT51000.045.1762965
GAGTAGC32150.044.899564
GTAGCTG52450.043.2020956
GCTGGGA109800.042.1238639
CCCAAAG70200.041.6689531
CCCGAGT17950.040.60941
CCAAAGT66450.040.4774252
TAGCTGG56850.040.4347277
AAAGTGC60500.040.4140784
TCGGGAG18100.040.258396
AAGTGCT62400.039.3342255
TTGGGAG76500.039.2683566
GGAGGCT91800.039.1188559
CTACTTG21150.038.685722
CAAAGTG67150.038.444853
Produced by FastQC (version 0.11.5)