FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806878
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9500.039.0802733
CCGAGTA9850.035.7997742
CTACTCG8100.034.8274352
TACTCGG8450.034.481733
GAGTAGC23900.032.641064
CCCGAGT11400.031.432641
AGTAGCT39100.031.1298565
GCTGGGA75250.031.1022959
GTAGCTG37400.031.0369786
TAGCTGG41300.030.3830097
GCTACTC19650.028.3133721
CCCAAAG51850.028.189351
CTCGGGA11400.028.032265
TCGGGAG11650.027.0273176
AAGTGCT43700.026.9927045
AAAGTGC43550.026.2223854
AGTGCTG46950.026.0250646
ACTCGGG12150.025.9150834
CCAAAGT51300.025.570672
CGGGTTC6500.025.3060429
Produced by FastQC (version 0.11.5)