FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074995
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC2101.8063147E-6149.86066270-279
TCGTCGA2050.0097672865130.24211330-339
GTCGTTA3506.258102E-6116.727066300-309
AGTAGCT99300.067.079075
CGAGTAG28550.064.5875553
GTAGCTG106450.062.5135846
GAGTAGC66150.062.017874
CCGAGTA30500.061.326582
TAGCTGG116100.058.802077
GCTGGGA226650.054.8370829
AAGTGCT105350.054.2636835
AAAGTGC113900.049.966274
CTACTCG25200.049.145322
TACTCGG25950.048.929733
CCAAAGT124450.048.4750062
CAAGTAG45550.047.906543
TTGGGAG133800.047.827826
AGTGCTG119400.047.824946
CCCGAGT40300.047.609361
CAAAGTG125200.047.2424553
Produced by FastQC (version 0.11.5)