FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074995
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT96050.063.483485
CGAGTAG29450.062.710923
CCGAGTA29800.061.975142
GAGTAGC65400.061.4778944
GTAGCTG101050.060.4480326
CTACTCG28500.059.7028962
TACTCGG28600.058.8582533
GCTACTC59500.056.695271
TAGCTGG111300.056.4502037
AAGTGCT112100.055.0963945
GCTGGGA227900.054.4162949
CGGTAAA4950.006709756352.96261245
AAAGTGC119350.052.409084
TTGGGAG144950.052.1640746
CCAAAGT129500.052.1361582
CCCAAAG146350.050.900381
CAAAGTG127250.050.487773
AGTGCTG124250.048.8688166
CCCGAGT38100.048.8002471
AACGGGT5400.00946404348.549057245
Produced by FastQC (version 0.11.5)