FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4785609
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT902.5354071E-531.4411531
CCCAAAG80950.029.6545031
GCTGGGA117650.029.5607769
GGAGGCT87150.026.4243779
AAAGTGC71200.026.4026174
TACTCGG11950.026.3495883
TTGGGAG73700.026.2722156
CCAAAGT82500.026.220362
CGAGTAG13950.025.9408073
CTACTCG11450.025.8743722
AGTAGCT56600.025.740225
AGTGCTG76900.025.301196
AAGTGCT74350.025.2840145
CAAAGTG81050.025.2233773
CCGAGTA15400.025.0396292
GCTACTC30150.025.0277841
GTGCTGG76450.024.8973757
CGGGTTC11200.024.7576859
GTAGCTG57700.024.6793526
GAGTAGC33800.024.4716324
Produced by FastQC (version 0.11.5)