FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4123581
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11650.034.307563
CTACTCG11850.034.125342
CGAGTAG12000.029.3884643
CCGAGTA11800.029.0895982
GCTACTC29050.028.6534351
GCTGGGA99250.028.650149
CCCAAAG72650.028.093361
CTCGGGA15600.027.1278115
TTGGGAG66650.026.7306466
GGAGGCT77850.026.4434039
GAGTAGC29650.026.325724
CCAAAGT71900.025.8324182
GTAGCTG48000.025.6584516
AAAGTGC62050.025.4621094
GGGAGGC89050.025.3346258
GTGCTGG66500.025.3034117
AGTAGCT47800.025.1830845
CCCGAGT15750.025.08121
AGTGCTG67450.025.0197456
CGGGTTC9250.024.897539
Produced by FastQC (version 0.11.5)