FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3840654
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG12850.042.459132
CCGAGTA14300.041.1140442
CGAGTAG13650.041.0044063
TACTCGG13250.040.1124153
GCTGGGA106000.037.8252759
GAGTAGC32700.036.6777764
AGTAGCT53000.036.029715
GCTACTC28500.035.984751
CCCAAAG77200.035.6488381
CCCGAGT17450.035.0473141
GTAGCTG54700.034.7262156
AAAGTGC62350.034.2475854
TTGGGAG72600.033.6767466
TAGCTGG58900.033.520227
AAGTGCT66150.032.9201435
ACTCGGG16750.032.85354
TCGGGAG18000.032.3901256
CCAAAGT74800.032.0060772
AGTGCTG69750.031.8845566
CAAAGTG73100.031.5278933
Produced by FastQC (version 0.11.5)