FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174594
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8250.037.0270543
CTACTCG8300.036.824882
GCTGGGA80250.031.0968259
CCGAGTA10250.030.7366812
CGAGTAG9950.030.700823
CTCGCGT1252.3672328E-730.0773624
TCGGGAG11450.029.1415486
CCCAAAG58750.028.3263021
AAAGTGC49750.027.86694
CCCGAGT13150.027.2465111
AGTAGCT39250.027.0600455
GTGCTGG55050.026.9771677
AAGTGCT51600.026.9588765
TCGCGTT700.0088421126.8547845
CTCGGGA12100.026.79935
AGTGCTG53450.026.553336
GCTACTC21200.026.4627021
TAGCTGG43050.026.2002037
CCAAAGT56800.026.1604252
GAGTAGC25550.026.1190364
Produced by FastQC (version 0.11.5)