FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174594
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10250.040.8321532
CGAGTAG10050.039.773063
CTACTCG11200.039.468072
TACTCGG11400.037.538133
CCCGAGT12700.036.292771
GCTACTC25950.031.8987051
GCTGGGA84650.031.8694539
GAGTAGC23450.031.684264
TCGGGAG15400.029.9160736
GGAGGCT65200.029.5546599
CTCGGGA15900.028.6877865
CCCAAAG60100.028.4072971
GTAGCTG40300.027.8799536
AGTGCTG55900.027.668356
GTGCTGG55900.027.6644297
ACTCGGG16200.027.5764184
TAGCTGG43850.027.4415077
AGTAGCT40700.027.267175
TTGGGAG57250.027.2622576
AAGTGCT54200.027.2428675
Produced by FastQC (version 0.11.5)