FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564509
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG4156.7466353E-660.49566230-234
CGAGTAG17200.057.8695263
CCGAGTA18950.054.7818342
CGACTAC3952.6918077E-454.664135240-244
AGTAGCT64700.052.3809435
CTACTCG20550.051.1108932
TACTCGG21250.050.8633653
TAGTCCG3008.935097E-450.2114230-234
GAGTAGC42450.049.340224
GTAGCTG68450.049.142726
CGCTAAA5050.00914324948.973766245
GCTGGGA157550.047.227979
AAGTGCT84450.046.1323625
TAGCTGG76400.045.3811077
GCTACTC48150.044.6450921
CCAAAGT95500.044.5043752
AAAGTGC87200.044.1873054
GGAGGCT122850.043.475739
CCCAAAG105900.043.1353151
TCGGGAG28750.043.106156
Produced by FastQC (version 0.11.5)