FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3825499
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT81900.049.277725
CTACTCG18150.048.525892
GTAGCTG85100.048.4207346
TACTCGG18500.046.523723
TAGCTGG89300.046.363037
GAGTAGC53250.046.2824174
GCTGGGA178350.046.2817849
CGAGTAG21300.044.6936153
CCGAGTA22250.044.56872
AAGTGCT92650.044.334215
CCAAAGT106150.041.6087882
AGTGCTG100600.041.543586
CAAAGTG105250.040.7639273
AAAGTGC98500.040.3111234
CCCAAAG119300.039.9913671
GCTACTC43300.039.092711
TCGGGAG26500.038.3815576
TTGGGAG102700.037.7737736
GGAGGCT126400.037.1394049
GTGCTGG112600.037.116687
Produced by FastQC (version 0.11.5)