FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3825499
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG2900.001187774281.97959245
CGAGTAG18600.052.9593243
CTACTCG22000.050.6513822
TACTCGG22100.049.865043
AGTAGCT70900.049.147335
GAGTAGC46500.048.8543364
CCGAGTA20800.048.5418052
GCTGGGA171450.046.952429
AAGTGCT93400.046.865415
GTAGCTG75550.045.8683136
AAAGTGC94700.045.7674
GCTACTC49600.045.5616381
CCAAAGT106800.044.559842
TTGGGAG113250.043.7518586
CAAAGTG103950.043.530963
TAGCTGG82150.043.3780567
AGTGCTG101650.043.355536
CCCAAAG119500.042.8716661
GGAGGCT138800.041.6798449
GTGCTGG110250.040.1932377
Produced by FastQC (version 0.11.5)