FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3234911
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10350.034.055213
CCGAGTA10750.033.2529682
CCCAAAG58650.030.715021
GCTGGGA83950.030.623129
CTACTCG7000.029.5651382
CCCGAGT14000.029.3053321
CCAAAGT54000.028.7438852
GTAGCTG40400.028.5001666
AAAGTGC49350.028.473884
AGTAGCT40200.028.0574285
GAGTAGC23950.027.864134
TAGCTGG43250.027.6013557
GCTACTC20000.026.8800621
AAGTGCT52600.026.7145635
AGTGCTG52250.026.7136236
GGAGGCT56300.026.0452799
CAAAGTG55500.025.996093
TACTCGG7600.025.9715793
GTGCTGG55100.025.7595427
CGTCGAA950.001305596124.7352294
Produced by FastQC (version 0.11.5)