FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933147
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG12250.056.8277782
TACTCGG13150.053.2961123
CGAGTAG13550.049.9871333
CCGAGTA15250.047.1907232
GCTACTC29850.045.5465241
AGTAGCT51900.044.5879975
CCCAAAG70850.042.827391
GAGTAGC32750.042.6552774
AAAGTGC56400.042.532284
CCAAAGT65250.042.314832
GCTGGGA112950.042.235159
ACTCGGG16400.042.160054
AAGTGCT59000.041.4544875
GTAGCTG54350.041.3530736
TAGCTGG57000.041.071927
CCCGAGT17850.040.586751
TCGCGAA704.638523E-640.2846994
CAAAGTG63950.040.0122873
GGAGGCT86750.039.9293489
TCGGGAG17850.039.775736