FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136677
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13900.048.348613
CTACTCG11000.048.3064042
CCGAGTA14250.047.5189252
TACTCGG11250.046.369583
AGTAGCT51600.043.8995485
GAGTAGC31700.042.9933664
GTAGCTG52100.042.4860046
CCCGAGT17050.042.3108021
GCTGGGA105650.042.0390329
TAGCTGG56150.040.7633257
GCTACTC26400.040.5420951
AAAGTGC56800.039.466884
CCCAAAG68850.039.103571
CCAAAGT63750.038.0617262
AAGTGCT60100.037.9253855
CAAAGTG63900.036.6996963
AGTGCTG62000.036.232556
ACTCGGG15000.035.717114
TCGGGAG17150.034.2537886
TTGGGAG67600.034.0652926
Produced by FastQC (version 0.11.5)