FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136677
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13850.050.5911372
TACTCGG14700.049.2653273
GCTACTC30850.048.4810831
CGAGTAG12900.046.2969063
CCGAGTA14250.044.550942
AGTAGCT46850.042.557925
CCCGAGT16500.041.6168561
GTAGCTG48950.041.3940436
AAAGTGC59550.041.2994274
AAGTGCT59150.041.0222135
GCTGGGA104150.041.0210769
CCCAAAG70050.040.9564251
CCAAAGT64500.040.0267752
GAGTAGC30350.039.0450484
GGAGGCT89050.038.3182039
CAAAGTG65400.038.1816523
TAGCTGG54750.037.8615537
TTGGGAG72850.037.7519656
AGTGCTG64850.037.117036
TCGGGAG19650.036.8443956
Produced by FastQC (version 0.11.5)