FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248711
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8150.031.1639862
TACTCGG7950.030.739633
GCTACTC19450.028.6122191
CGAGTAG9500.027.2083073
CCGAGTA10350.026.8119562
GCTGGGA75550.026.4372889
CCCAAAG58250.025.4227661
CCCGAGT12800.024.6861821
CCAAAGT53450.023.9352532
TAGCTGG39650.023.5869797
AAAGTGC45450.023.4722334
GGAGGCT57400.022.924969
GAGTAGC24250.022.8682384
AGTAGCT38100.022.819665
GTAGCTG38550.022.6751966
CGGAGGT10500.022.379137
GTGCTGG48000.022.323187
AAGTGCT48150.022.2536375
AGTGCTG50250.021.884786
CTCGGGA11600.021.8775255
Produced by FastQC (version 0.11.5)