FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248711
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8100.029.611653
ACGCGTA404.7855475E-429.37264884-85
GAGTAGC22700.028.7978274
AGTAGCT37600.028.7680745
GTAGCTG37500.028.5842726
CTACTCG9050.028.0622622
GCTGGGA82100.028.0515049
TAGCTGG40200.027.713997
TACTCGG9450.027.3721163
CCGAGTA9150.027.2415772
CCCAAAG59400.026.2887121
GGAGGCT59550.025.0985459
AAGTGCT50650.025.0700445
AAAGTGC50200.024.5453014
AGTGCTG53050.023.6618926
GTGCTGG54850.023.6542517
TCGGGAG13650.023.4206986
TTGGGAG50250.023.3898546
CTCGGGA12750.022.8692685
CCAAAGT59350.022.584042
Produced by FastQC (version 0.11.5)