FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA93150.031.9362419
CTACTCG9700.031.5135672
CGAGTAG11050.031.0459443
CCGAGTA12450.030.9742242
CCCAAAG63850.030.3431421
AGTAGCT45800.030.1665845
TAGCTGG48900.029.8892147
TACTCGG10100.029.3132173
GTAGCTG46200.029.091656
CCAAAGT62150.028.148662
GAGTAGC29950.028.0866874
TTGGGAG57050.028.0070656
AGTGCTG55900.027.7425526
AAAGTGC56600.027.3994484
GCTACTC23250.027.3709871
GTGCTGG56650.027.0446077
GGAGGCT66600.027.026369
AAGTGCT57850.026.9698815
CGGAGGT12350.026.6375377
CCCGAGT16000.026.5156421
Produced by FastQC (version 0.11.5)