FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485377
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11950.042.8963432
TACTCGG12300.042.4404073
GCTACTC30000.034.4965251
GCTGGGA91300.034.491849
CTCGGGA16500.033.0615545
TCGGGAG17050.032.5359466
AGTAGCT43550.032.071375
GTAGCTG43800.031.9851196
GGAGGCT80850.031.7413169
CCGAGTA11300.031.6298272
TTGGGAG67100.031.3177686
CGAGTAG11400.030.9398423
ACTCGGG16450.030.875454
GAGTAGC28100.030.7940584
CCCAAAG67600.030.7573931
CCAAAGT63550.030.1932
AAAGTGC55300.030.1896924
TAGCTGG50150.030.0877727
CCCGAGT14000.029.2324471
AAGTGCT59050.028.8295525
Produced by FastQC (version 0.11.5)