FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058356
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9300.031.3547423
CTACTCG9000.030.8321592
GCTGGGA69950.028.4220759
CGAGTAG7900.027.9811273
CTCGCGT909.48433E-426.1285234
GAGTAGC20950.025.367744
CCCAAAG52150.025.2575131
TTGGGAG47800.025.179686
GGAGGCT54350.025.07859
CTCGGGA12700.024.8118115
TCGGGAG12950.024.687516
AGTAGCT34600.024.60315
CCGAGTA8650.024.4675712
GCTACTC22900.024.4454671
AAGTGCT45800.024.1318025
GGGAGGC64750.023.8817628
AAAGTGC45150.023.854214
GTAGCTG34750.023.8120066
CGGAGGT10950.023.6106827
ACTCGGG13050.023.425574
Produced by FastQC (version 0.11.5)