FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632031
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6800.044.950573
CTACTCG6750.042.4968572
CCGAGTA8500.038.173412
CGAGTAG8400.037.5082053
CCCGAGT10050.035.1007841
GCTGGGA60950.033.3904349
GCTACTC17100.032.7319951
GAGTAGC18750.032.604154
TCGGGAG9450.031.837476
CTCGGGA8750.031.708435
GTAGCTG31350.031.6399146
CCCAAAG46150.031.084921
GGAGGCT50600.031.0244319
AGTAGCT30800.030.6885325
TAGCTGG32900.030.289297
ACTCGGG10900.029.7682554
CCAAAGT45650.029.0495282
CGGAGGT11250.028.830077
AAGTGCT39800.028.7113745
AAAGTGC40000.028.5678144
Produced by FastQC (version 0.11.5)