FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661008
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9250.044.2010843
CCGAGTA10000.043.7353132
TACTCGG9000.042.2958643
CTACTCG9000.041.801972
GAGTAGC20950.040.377914
CCCGAGT11350.039.8986051
GCTGGGA77350.038.945129
GCTACTC20000.037.9732931
CCCAAAG54200.037.9463121
AGTAGCT34450.037.5144775
GTAGCTG35550.036.7502756
TAGCTGG36950.035.9937747
AAAGTGC45000.035.8209424
CCAAAGT52500.035.382382
AAGTGCT46650.034.5539635
GTGCTGG50400.033.1951687
CAAAGTG50600.032.9710853
TACGGCG1001.4131674E-632.8967864
TCGGGAG13400.032.2655036
AGTGCTG49700.032.2443356
Produced by FastQC (version 0.11.5)