FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681527
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10050.038.374782
TACTCGG10700.036.0436023
GCTACTC24800.031.6766821
CTCGGGA13500.028.2187395
CCGAGTA8700.027.5707822
TCGGGAG14350.027.1923796
GGAGGCT67900.026.6508879
CGAGTAG8450.026.1600933
GCTGGGA82750.025.844269
CCCAAAG61200.025.8263321
ACTCGGG15200.025.3724564
CGGAGGT12650.025.2699287
TTGGGAG57450.024.8773776
GAGTAGC23650.024.8582784
AAAGTGC52650.024.8335424
CTCGCGT1151.3716884E-424.5383664
GGGAGGC74150.024.531638
AGTAGCT37550.024.4236765
GTAGCTG39700.024.1579846
TAGCTGG41100.023.447567
Produced by FastQC (version 0.11.5)