FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338479
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7250.036.9711342
TACTCGG7650.034.402683
GCTACTC19950.030.7093871
GCTGGGA75500.030.1284719
CCCAAAG55700.029.443861
CCGAGTA10750.028.4335332
CGAGTAG10050.028.0576233
GGAGGCT58000.027.2263859
AAAGTGC48400.026.5083664
AGTAGCT37700.026.053775
CCAAAGT55800.025.956282
GTAGCTG38150.025.8696446
TAGCTGG41800.025.7476677
CCCGAGT12900.025.5728441
GAGTAGC24150.025.298334
AAGTGCT50550.025.1019975
TCGGGAG11850.024.985496
GTGCTGG50050.024.6962157
TTGGGAG52700.024.6129766
CAAAGTG55950.024.2752343
Produced by FastQC (version 0.11.5)