FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338479
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT651.4105257E-436.1692545
TACTCGG10600.032.3822063
CTACTCG9800.031.6670272
TTGGGAG54600.029.702896
GCTGGGA81100.029.672659
CGAGTAG9350.029.670843
GGAGGCT62900.029.590469
GAGTAGC23200.029.387964
CGGAGGT12500.029.3303877
CCGAGTA9200.029.1324142
CCCAAAG56050.029.119241
AGTAGCT37550.028.8005545
GGGAGGC71900.028.3051998
GTAGCTG38750.028.1441786
CTCGGGA12950.027.231685
GCTACTC22450.027.0271031
TAGCTGG42800.026.5769317
AAAGTGC49150.026.4999874
TCGGGAG14150.025.9125696
AAGTGCT51150.025.5553635
Produced by FastQC (version 0.11.5)