FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3563065
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9800.030.2295913
CTACTCG8950.029.9481242
GCTGGGA85250.029.3857789
GCTACTC22950.029.0998381
AGTAGCT40450.028.9462625
CCGAGTA11550.028.0921442
GAGTAGC26900.027.6195454
CGAGTAG10750.027.5581383
GGAGGCT64600.026.7743959
GTAGCTG41900.026.7062956
CCCAAAG61550.026.132621
TTGGGAG57750.026.0524586
AAAGTGC48650.025.1305624
TAGCTGG47150.025.1247887
CCCGAGT14900.024.6202931
CTCGGGA12800.024.6136785
CCAAAGT56650.024.5702512
AGTGCTG54250.023.7466036
GTGCTGG55600.023.504647
AAGTGCT52900.023.4671295
Produced by FastQC (version 0.11.5)