FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192192
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10650.049.022
TACTCGG11000.048.3154143
CGAGTAG11550.046.8290983
CCGAGTA12400.046.6534082
CCCAAAG65800.041.1779441
CCCGAGT14500.040.5517921
GCTGGGA100000.039.9984869
AGTAGCT44000.039.6564985
GAGTAGC28850.039.28844
AAGTGCT58850.038.4407425
CCAAAGT62300.038.1999172
GTAGCTG47050.037.971746
AAAGTGC57000.037.8731084
TCGGGAG16200.037.4378786
AGTGCTG62300.037.0535626
TAGCTGG50750.036.5866557
GTGCTGG63000.036.3370827
CAAAGTG62900.035.8166353
GGAGGCT75200.035.813839
TTGGGAG65300.035.3512546
Produced by FastQC (version 0.11.5)