FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156111
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11250.038.038932
TACTCGG11300.037.430123
CGAGTAG12950.037.378743
CCGAGTA13400.035.094142
GCTGGGA100950.033.93829
AGTAGCT53450.031.7407135
GAGTAGC32850.031.473464
GTAGCTG54200.031.128086
GCTACTC27800.030.872011
CCCAAAG71700.030.7139761
CGGGTTC8500.030.4096479
CCCGAGT16700.030.2138181
TAGCTGG57850.029.4897377
CCGGGTT9250.029.468228
CTCGGGA14950.029.234725
GGAGGCT75800.028.7064469
AAAGTGC63900.028.3885774
TCGGGAG16050.028.1095166
TTGGGAG68800.027.5279466
CGGAGGT14000.027.1909627
Produced by FastQC (version 0.11.5)