FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086342
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9150.031.3561552
GCTGGGA93300.031.179729
TACTCGG9550.031.0016233
GAGTAGC28050.029.8218364
CCGAGTA11550.029.7272642
AAAGTGC59000.029.3118384
TCGGGAG13550.029.1331376
AGTAGCT46100.029.0530155
CCCAAAG72700.028.6695081
GCTACTC23650.028.5126321
CGAGTAG11900.028.038753
AAGTGCT61700.027.9529825
TAGCTGG49950.027.0969377
GTAGCTG47950.026.8540136
GGAGGCT65900.026.6716219
CCAAAGT69050.026.5653152
AGTGCTG65150.026.1120766
CTCGGGA13400.025.95225
CCCGAGT15450.025.9431571
GTGCTGG65650.025.770987
Produced by FastQC (version 0.11.5)