FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745757
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1452.5867848E-829.2602331
CTACTCG7900.026.7846032
TACTCGG7800.026.5102793
TCGGGAG10750.024.9185436
CTCGGGA9700.024.2244835
GCTACTC20650.023.2853811
CCGAGTA9400.022.0102312
CGAGTAG8900.021.6496093
GCTGGGA61300.021.6203279
CCCGAGT11700.021.3546871
GGGAGGC51750.020.252018
GGAGGCT49550.020.2027059
ACTCGGG10800.020.01664
GAGTAGC20300.019.6779174
ACGTCGA1250.006303465518.8565921
GGCGGAG11600.018.6361455
CCCAAAG53050.018.3056471
CGGGAGG15050.017.7996717
GCGCAAA1600.001253802917.6780571
TTGGGAG45500.017.6620436
Produced by FastQC (version 0.11.5)