FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745757
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA352.938391E-453.7142079
TATCGCG450.00101312441.7872056
TCGCGTT851.7480059E-533.1981475
CTCGCGT1452.6466296E-829.1914714
TACTCGG8350.024.7829783
CGGCACG1802.1497362E-723.5156692
CGCGTTG1201.8363145E-423.5053046
GCTGGGA65350.022.4391429
GGCGGAG13100.022.2588085
CTACTCG8900.022.1945632
GCTACTC20550.021.9740731
GGAGGCT50300.021.4910249
CCCAAAG54150.021.1954441
CCGAGTA8550.020.3527412
CGAGTAG8900.020.0807953
CGGAGGT9800.019.6650757
AGGCGGA13750.019.4963674
TTGGGAG44600.019.3945086
TCGCCGT2451.9653635E-719.18415894
CCCGAGT11050.019.155851
Produced by FastQC (version 0.11.5)