FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607834
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA2650.055.160771
CGTCGAG4900.046.191241
CGCGACT952.0589141E-839.708261
GTCGAGA10150.029.7322941
CCGAGTA7750.022.4551932
CGAGTAG8450.021.690193
CCCGAGT11650.019.4280741
GCTGGGA70800.018.3876429
CGAGACT22900.017.7083231
TCGAGAC18800.017.262672
GCTACTC17950.017.0751041
GAGTAGC22950.016.5866174
CCCAAAG55800.016.4783941
TACTCGG7150.016.4319633
GACTTGT33200.016.1369784
TAGCTGG42150.016.0562637
AGTAGCT37200.015.7914695
ACGGGCG1800.002812425815.6660058
GTGATCA34300.015.4833129
CGGAGGT8650.015.2132317
Produced by FastQC (version 0.11.5)