FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4262433
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG1104.787489E-470.8963280-289
AGTAGCT106850.066.095525
CGAGTAG31650.064.3084953
GAGTAGC71150.063.0247274
GTAGCTG108850.062.9815446
CCGAGTA33450.061.459172
TAGCTGG114650.060.754897
CTACTCG26550.060.386922
TACTCGG27350.059.0628173
AAGTGCT116100.057.9911735
GCTGGGA237750.057.3453879
GCTACTC56100.055.753161
AAAGTGC124600.053.879544
CCAAAGT133200.053.4365962
CAAAGTG132250.052.326933
CCCAAAG154750.050.8848081
TATCGTA3900.00796508650.718647340-345
AGTGCTG130000.050.4485976
CCCGAGT42950.048.699511
CAAGTAG47600.048.5967833
Produced by FastQC (version 0.11.5)