FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4262433
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA1102.3582845E-4123.12693225-229
GTCCGAT3903.8938662E-580.78312245
GCGAATT3200.001798933573.84082245
CGAGTAG26800.064.879113
AGTAGCT95050.064.4423455
CTACTCG32050.063.388582
CCGAGTA27800.061.886962
GAGTAGC60800.061.8111574
TACTCGG33650.061.2810823
GTAGCTG101300.061.170426
AAGTGCT114650.059.332215
GCTACTC67900.059.0441781
AAAGTGC119150.057.2961924
TAGCTGG109650.057.0650757
GCTGGGA226400.056.082969
CCAAAGT129950.054.7884942
CAAAGTG128050.054.315043
TCGGGAG42550.053.3212746
CGTCGAC2650.006684055653.02083230-234
CCCAAAG148850.052.9247631
Produced by FastQC (version 0.11.5)