FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4255375
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12400.029.528212
CGAGTAG12300.028.6233433
CCCGAGT15850.023.7376141
GCTGGGA93450.022.3526789
GAGTAGC29750.022.2480134
CGCCGTA2354.9385562E-922.12192794
TACTCGG10050.021.9531063
AGTAGCT48450.021.7990535
CTACTCG10150.020.811852
TAGCTGG52750.020.6449787
GTAGCTG52600.020.4361296
CCCAAAG74200.018.4444391
AAAGTGC60800.017.6803574
AAGTGCT64450.017.6254965
TCGCCGT5100.017.60684894
GGAGGCT67200.017.6027369
TTGGGAG63450.017.3854186
CCAAAGT69000.017.2802032
GCTACTC26700.017.2620031
GTGCTGG63800.017.1428937
Produced by FastQC (version 0.11.5)