FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264501
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT86200.067.41655
CCGAGTA26700.067.147882
CGAGTAG25500.065.458973
GTAGCTG92300.061.9167946
GAGTAGC60650.059.800694
TAGCTGG102050.057.6118437
GCTGGGA195150.054.460399
AAGTGCT93200.051.9608545
CCCGAGT36950.048.93031
CAAGTAG39050.048.19843
CCAAGTA42000.046.4485472
AAAGTGC103900.046.0147174
AAGTAGC42800.045.9013374
CCAAAGT111750.045.6099322
AGTGCTG105250.045.5743066
CAAAGTG110150.044.8381273
CCCAAAG123750.044.6629561
TGAGTAG43750.044.2765853
TTGGGAG113050.042.5510866
CTGAGTA47000.041.6533622
Produced by FastQC (version 0.11.5)